期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2013
卷号：2
期号：8
页码：3563
出版社：S&S Publications
摘要：Laccase is copper containing polyphenol oxidase that acts on wide range of substrates. It is also known as“Green Catalysts” or “Ecofriendly” enzymes. Laccase has many potential applications including textile dyedecolourization, delignification of pulp and effluent detoxification. In the present study, the cyanobacterial strains werescreened for laccase production and dye decolorization potential of crude laccase were investigated. Among all selectedstrains Lyngbya NCCU-102 showed activity (34 mU/ml), Synechocystis NCCU-370 stood at second position (31 mU/ml).Maximum laccase production was found on 7th day. Laccase activity of studied strains ranged from was 24.9 to 34 mU/ml.In all strains under normal condition cyanobacteria showed low laccase activity but in addition of inducer (guaiacol) it wasamplified multiple time (60%-80%). Though, under normal condition highest laccase activity was exhibited bySynechocystis NCCU-370 but highest laccase activity induction was shown by Lygbya NCCU-102. The crude laccase ofLyngbya and Synechocystis revealed a promising result on the decolorization of synthetic dyes. About 74.1% of reactiveblue 4 were effectively decolorized by Synechocystis and 59.45% by Lyngbya within 144 hr of incubation. This is firstreport of freshwater cyanobacteria for the laccase production and its decolorization ability and this will provide a possibleway to solve environmental problems.
关键词：Laccase; guaiacol; Lyngbya NCCU-102; Synechocystis NCCU-370; Reactive blue 4; Decolorization.
