期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2013
卷号：2
期号：9
页码：4328
出版社：S&S Publications
摘要：Present study screened ten Cyanobacterial extracts for antimicrobial activity, cytotoxicity (against humancervical carcinoma cells Hela and SiHa) & GC-MS for chemical composition. Cyanobacterial extracts were subjectedto Agar Well Diffusion Assay at concentration of 100μg. well-1 and incubated at 37 + 2oC. Inhibition zone wasmeasured in millimetres (mm) after 18-24 hour. Minimum inhibitory concentration (MIC) was determined by using thebroth microdilution method in 96-well microtitre plates. Drug dilutions were performed using cation adjusted MuellerHinton Broth (MHB) in a concentration range of 128-0.25 μg.ml-1. Cytotoxicities were assessed using MTT assay. GCMSanalysis was carried out on a GCMS-QP 2010 Plus Shimadzu system having RTX-5 column (Restek, USA) (60 m,ID 0.25mm, film thickness 0.25 μm). Cyanobacterial extracts exhibited significant antibacterial effect on clinicalisolates of S.aureus MRSA & MRSE whereas selectively inhibited Gram-negative. Minimum inhibitory concentrations(MIC) were in the range between 64 to 128 μg/ml. Activity Index of active extracts ranged from 0.33 to 1.50. ActivityIndex and Zone of Inhibition were significantly correlated with p>0.03.GC-MS detected distinct groups of activecompounds with pronounced presence of saturated and unsaturated fatty acids. Pharmaceutically important compoundslike sesquiterpenoids (Farnesol), dicarboxylic acids, imidazole, indolinones, α-tocopherol, phenolics, phytosterols,heptadecane, tetradecane and 9-octadecenal were moderately present. Extracts also exhibited cytotoxic effect on humancervical carcinoma cells lines with LD50 values ranging from 34 to 146μg/ml. Cyanobacterial species have distinctactive group metabolites which are promising sources of antiproliferative and antimicrobial compounds.
