期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2013
卷号：2
期号：9
页码：4556
出版社：S&S Publications
摘要：Diabetes Mellitus is a metabolic disorder characterized by high blood sugar level caused due to deficiency of insulin secretion or insulin action. One of the therapeutic approach to treat Type II Diabetes is to lo wer the postprandial blood glucose level by inhibition of carbohydrate hydrolyzing enzyme such as alpha-amylase. In present investigation polyherbal formulation (PHF) composed of 22 medicinal p lants having anti-diabetic property were selected from WHO mo no graphs and evaluated for in vitro alpha-amylase inhibitory activity. Air dried powders of 22 medicinal plants were divided into four categories. One formulatio n named as(PHF1) was used and prepared by mixing the powders in an optimized ratio of 80:10:5:5 .Extracts of PHF1 prepared by soxhlet method using po lar and non-polar solvent was subjected to Inhibition assay b y using Dinitro salicylic acid (DNS) method and phytochemical constituents in the extract was analyzed qualitatively as well as by GC-MS.The results revealed the presence of glycosides, steroids, terpenoids, saponins, phenols and tannins. In vitro study indicates that PHF1 Hot water extract showed maximum percentage inhibitionof alpha- amylase activity. This hot water extract of PHF1 can be effective in lowering postprandial hyperglycemia (PPHG).
