期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2013
卷号：2
期号：9
页码：4847
出版社：S&S Publications
摘要：Different concentrations and combinations of hormones were used in MS medium to observe callus inductionand then plant regeneration using leaf as explant. The rate of callus formation varied in different treatments used. Thehighest amount of callus (80.67%) was produced on MS medium containing 3.0 mg/l NAA from leaf after 20 days. Thehighest fresh weight of callus was obtained from 2.0 mg/l IAA and 0.5 mg/l BAP. The highest percentage of regeneration(46.28%) was recorded in MS media containing 2.0 mg/l BAP + 0.25 mg/l IAA from leaf after 42 days. The protocol in thestudy might be useful for the production of disease free and healthy plant materials and also it would be useful for genetictransformation and secondary metabolite production of Physalis minima using biotechnological approach.
