期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2013
卷号：2
期号：10
页码：5283
出版社：S&S Publications
摘要：Rohu (Labeo rohita) is a species of fish of the carp family cyprinidae. A survey was carried out fromSeptember, 2012 to August, 2013 to study the prevalence of bacterial ulcerative disease in L. rohita, cultivated in afreshwater farm located at Moongilthuraipattu Village of Villupuram District, Tamil Nadu, India. To mimic theinfection in healthy fish, organs such as liver, gill and ulcerative skin were used to prepare the inoculum in PBS buffer.The inoculum was injected into healthy animals through intramuscular injection, immersion challenge and oral route.The tissue supernatant from infected rohu fish was screened for isolation and screening of the causative organismresponsible for ulcerative lesion in L. rohita. Based on the biochemical, morphological and molecular features thebacterial culture isolated from infected rohu was tentatively identified as Providencia species like bacterium which wasfurther confirmed by the analysis of its 16S rRNA gene using PCR and DNA sequencer and it has been identified as P.stuartii. Further, its partial sequence was deposited in GenBank (KF155520.1).
