期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2013
卷号：2
期号：10
页码：5377
出版社：S&S Publications
摘要：Globally environmental scientists have shown increased interest in promoting biological methods referred to as “Green technology”in the clean-up technologies for hydrocarbon-polluted sites. These methods are less expensive and do not introduce additionalchemicals/pollutants into the environment. Compared to physico-chemical methods, bioremediation / biodegradation offer a feasible alternativefor this purpose to the petroleum industry. One reason is that the majority of the molecules in the crude oil and refined products arebiodegradable. We have reported the isolation of Hydrocarbon Utilizing Bacteria (HUB) from crude oil contaminated soil and UntreatedEffluent water (UEW) samples collected samples from selected ONGC sites in Gujarat state. Screening for hydrocarbon utilizing capacity ofthese bacteria was carried out using Bushnell-Hass (BH) medium with crude oil (K#X) and redox indicator 2, 6 - dichlorophenol indophenol(DCPIP). Maximum utilization of hydrocarbons from crude oil was indicated by the total discoloration of DCPIP. In total five consortia(Hydrocarbon Utilizing Bacterial Consortium: HUBC) named HUBC-S, HUBC-W, HUBC, HUBC-Ank, and HUBC-AH were prepared toscreen for the most efficient consortium for hydrocarbon degradation. Our results indicated that the consortium HUBC consisting of sixisolates showed best results. Total discolouration of DCPIP by “HUBC” and its individual isolate was about 53 h and 120 h, respectively. Theresults of this study are presented here.
