期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2013
卷号：2
期号：11
页码：6032
出版社：S&S Publications
摘要：Intracranial infusion of neuroactive chemicals is routine method in neurophysiology research. We devised asimple, durable and reusable internal cannula for this purpose. The guide cannula, placed securely on the skull usingDental acrylic and securing screw, was fabricated from a needle of 22 gauge hypodermic needle. The internal cannulawas fabricated from 30 gauge Dental needle, which has a white coloured hub. The length of this internal cannula willbe enough to reach the nucleus when inserted through the guide cannula at the time of infusion process. We infusedOrexin A into BLA by using this cannula assembly in overnight fasted rats, which increased the food intakesignificantly. On histological examination we found that the location of injection was as desired in the BLA. The guidecannula and the internal cannula fabricated by us appeared to be highly dependable. The dental needle has high temperand it does not change the shape like insulin needle with limited reusability. We conclude that this method offabrication of internal cannula will be a better alternative to those fabricated from hypodermic needles.
