期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2013
卷号：2
期号：12
页码：7205
出版社：S&S Publications
摘要：An efficient regeneration system, direct and indirect organogenesis, was developed for Curculigo orchioides-an endangered medicinal herb. Shoot multiples were initiated from in vitro grown leaf explants through direct organogenesis on Murashige and Skoog's (MS) medium containing ½ strength nitrogen salts and 0.44 μM BA. Almost 10 shoots were obtained per leaf explant (1 cm long). On the other hand MS medium with full strength nitrogen salts and 2.22 μM BA, stimulated callus formation and almost 8 shoots were obtained per leaf explant (1 cm long ). Comparative study of shoots regenerated directly or through callus revealed differences in number of leaves, shoot length, root length, fresh and dry weight.
关键词：Curculigo orchioides; Kali-musli; Regeneration; Organogenesis and Leaf explant
