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  • 标题:Molecular Characterization of Pathogenic and Non-pathogenic Pseudomonas aeruginosa with Special Reference to Phenazine Gene
  • 本地全文:下载
  • 作者:Sundararaj Jamunadevi ; Pannerselvam Balashanmugam ; Gangatharan Muralitharan
  • 期刊名称:Journal of Modern Biotechnology
  • 印刷版ISSN:2319-6645
  • 出版年度:2012
  • 卷号:1
  • 期号:2
  • 页码:70-74
  • 出版社:Madras Institute of Biotechnology
  • 摘要:In the present study phenazine gene (Phz gene) amplification was carried out for the differentiation and comparison of Pseudomonasaeruginosa pathogenic form and commensal isolates. A total of 40 isolates were collected from different sources in differenthospitals around Tiruchirappalli region. Antibiotic sensitivity of the pathogenic organisms was done with 10 different antibiotics andshowed all the organisms were sensitive to imipenem and carbenicillin and resistant to ampicillin and tetracycline. Interestingly, allthe commensal isolates were resistant to pipercilliln, cefotaxime. Polymerase chain reaction amplified Phz gene in pathogens andcommensal revealed that phenazine gene was amplified only in pathogenic isolates but not in commensal. This study is shed light onthe possible application of this phz gene for rapid differentiation and comparison of pathogenic and non-pathogenic strains ofPseudomonas aeruginosa from various samples.
  • 关键词:Antimicrobial resistance; Pseudomonas aeruginosa; Phenazine pigment; Molecular characterization.
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