摘要：In the present study, 15 isolates of actinomycetes were isolated from upland paddy of Jeypore, Odissa. They werescreened for their antagonistic activity against four different fungal plant pathogens namely, Rhizoctonia solani,Helminthosporium oryzae, Curvularia lunata and Fusarium orysporum by dual plate assay. The antagonistic effectswere more prominent after two days in R. solani and three days in the other fungi. Act 8 and 10 resulted in an inhibitionof 1.1–1.5 cm, whereas others showed a minimum inhibition of <0.8 cm. The colony morphology of actinomycetes wasobserved under phase contrast microscope and it revealed the highly coiled spiral type of spore chains particularly inAct 7, 9 and 11. Genomic DNA was isolated from the selected isolates and they were amplified using PCR primers fD1and rP2, which produced amplification of 1500 bp. RFLP was used to analyze the genetic diversity of theactinomycetes isolates from the soil using restriction enzyme Sau3A, which formed restriction pattern producing bandswith 540–650bp confirming the isolates were belongs to the genes Streptomyces.
