It is very common that stored blood and various replacement solutions are simultaneously administrated during operation. The stored blood of healthy man was used to find out the hemolysis due to various replacemen solutions for 21 days. We used for the test solutions which were 5% Dextrose in Water, 5% Dextrose and Saline, N-Saline, Volex, Rheomacrodex-D and Hartmann's solution. The results are as follows: 1) It was very significant that F-Value of all the replacement solutions was 8,4773. 2) The most prominent effect of hemolysis was in Rhemacrodex-D and the least one was hartmann's solution among the six replacement solutions for 21 days. 3) It was shown the peaked hemolysis aroun 15th day in the six replacement solutions.