期刊名称：ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology
印刷版ISSN：2146-0213
出版年度：2012
卷号：2
期号：2
页码：101-117
DOI：10.18036/btdc.35526
语种：English
出版社：Anadolu University
摘要：The present study was designed to test the hypothesis that cholesterol rich diet-induced hypercholesterolemia in rabbits can be reversed by long-term treatment of antioxidants (proanthocyanidin and silymarin) and antihyperlipidemic drug(simvastatin). In this study 35 adult New Zealand White (NZW) male rabbits were used and divided into five groups. The rabbits in Group 1(control) was fed with rabbit chow diet. Group 2 was fed with only cholesterol (2% w/w) rich diet to induce hypercholesterolemia. The other groups received GPSE 100mg/kg (Group 3), silymarin 10mg/kg (Group 4) and simvastatin10mg/kg (Group 5) with cholesterol in addition to rabbit chow diet. At the end of the experiment, the animals were sacrified and their aorta were excised for intimal lesion analysis. Vessells were dissected, trimmed, and then placed fixed in neutral formalin. All tissue sections were stained with hematoxylin and eosin(H&E). Apoptotic process was detected by using TUNEL. Cholesterol treatment produced a sustainable state of atheromatous plaque formation In addition, only cholesterol rich diet-induced animals have shown a considerably high number apoptotic cells by using TUNEL and plasma total cholesterol (TC) and low density lipoprotein (LDL) levels were found.
