摘要：OBJECTIVE: The aim of this in vitro study was to investigate the effect of pulpal perforation and Ankaferd Blood Stopper (ABS) treatment on the bond durability to dentin of a self-etch adhesive system. MATERIALS AND METHOD: Forty non-carious extracted human molars were selected for microtensile bond strength test. Class I cavities (buccolingual width: 3 mm, mesiodistal width: 4 mm, depth: 3 mm) were prepared and specimens were randomly assigned to two main groups, one group with and the other without pulpal perforation. Half of the specimens in each group were treated with ABS and all specimens were restored with Clearfil Protect Bond (CPB) and a composite resin (Clearfil Photo Posterior). Then the teeth in all groups were assigned to two for 24 h and 12 month periods. Specimens of the 12 month group were stored in a moist condition under a simulated pulpal pressure of 15 cmH 2 O. After storage, all specimens were subjected to a microtensile bond strength test. The data were analyzed by using Kruskal-Wallis and Mann Whitney U tests. Interface morphology of the groups was examined by using scanning electron microscopy (SEM). RESULTS: Statistically significant differences were found between the ABS-treated and non-treated groups at either period (Bonferroni correction; p<0.0083); ABS reduced the microtensile bond strength to dentin of CPB. No significant differences were found between groups with and without pulpal perforation at both periods (p>0.0083) CONCLUSION: In a simulated pulpal pressure system, no significant differences were found between the bond strength of specimens tested after a storage of 24 h or 12 months. However, ABS reduced the microtensile bond strength of CPB to dentin. When using ABS, dentin contamination should be avoided.
