期刊名称：The Journal of Health, Population and Nutrition
印刷版ISSN：1606-0997
电子版ISSN：2072-1315
出版年度：2014
卷号：32
期号：4
页码：580-586
语种：English
出版社：International Centre for Diarrhoeal Disease Research Bangladesh
摘要：Shigellosis presents with varied clinical features are dictated by the species involved, virulence factors of the strain, and the host immune status. We studied the species, virulence genes, and antibiotic susceptibility pattern of the Shigella strains isolated from 33 children aged less than 12 years, with clinical features of shigellosis. Identification and antibiotic sensitivity of Shigella species were done using disc diffusion and E-test. Multiplex PCR was done for the detection of virulence genes ( ipa H, ial , set 1A, set 1B, sen , and stx ) and ESBL genes. Parents of the children were interviewed using structured questionnaire to assess the severity of the disease; 26 (79%) of the isolates were Shigella flexneri. Ciprofloxacin and ceftriaxone resistance was seen in 23 (69%) and 3 (9%) Shigella isolates respectively. Two ceftriaxone-resistant strains were found to harbour bla CTX gene and the third bla TEM gene. Virulence gene ipa H was detected in 100% of strains while ial , sen , set 1A, and set 1B were detected in 85%, 61%, 48%, and 48% respectively. Key words: Children, CTX-M, ESBL, Shigellosis, TEM, Virulence genes
