期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2016
卷号：113
期号：38
页码：10577-10582
DOI：10.1073/pnas.1607334113
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The human mitotic arrest-deficient 2 (Mad2) binding protein p31comet participates in the spindle checkpoint and coordinates cell cycle events in mitosis although its function in meiosis remains unknown in all organisms. Here, we reveal P31comet as a synaptonemal complex (SC) protein in rice (Oryza sativa L.). In p31comet, homologous pairing and synapsis are eliminated, leading to the homologous nondisjunction and complete sterility. The failure in loading of histone H2AX phosphorylation (γH2AX) in p31comet, together with the suppressed chromosome fragmentation in rice completion of meiotic recombination 1 (com1) p31comet and radiation sensitive 51c (rad51c) p31comet double mutants, indicates that P31comet plays an essential role in double-strand break (DSB) formation. Interestingly, the dynamic colocalization pattern between P31comet and ZEP1 (a transverse filament protein of SC) by immunostaining, as well as the interaction between P31comet and CENTRAL REGION COMPONENT 1 (CRC1) in yeast two-hybrid assays, suggests possible involvement of P31comet in SC installation. Together, these data indicate that P31comet plays a key role in DSB formation and SC installation, mainly through its cooperation with CRC1.
