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  • 标题:Neural tube morphogenesis in synthetic 3D microenvironments
  • 本地全文:下载
  • 作者:Adrian Ranga ; Mehmet Girgin ; Andrea Meinhardt
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:2016
  • 卷号:113
  • 期号:44
  • 页码:E6831-E6839
  • DOI:10.1073/pnas.1603529113
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Three-dimensional organoid constructs serve as increasingly widespread in vitro models for development and disease modeling. Current approaches to recreate morphogenetic processes in vitro rely on poorly controllable and ill-defined matrices, thereby largely overlooking the contribution of biochemical and biophysical extracellular matrix (ECM) factors in promoting multicellular growth and reorganization. Here, we show how defined synthetic matrices can be used to explore the role of the ECM in the development of complex 3D neuroepithelial cysts that recapitulate key steps in early neurogenesis. We demonstrate how key ECM parameters are involved in specifying cytoskeleton-mediated symmetry-breaking events that ultimately lead to neural tube-like patterning along the dorsal–ventral (DV) axis. Such synthetic materials serve as valuable tools for studying the discrete action of extrinsic factors in organogenesis, and allow for the discovery of relationships between cytoskeletal mechanobiology and morphogenesis.
  • 关键词:organoid ; extracellular matrix ; neural tube ; development ; embryonic stem cell
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