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标题：Conversion of Waste Agriculture Biomass to Bioethanol by Recombinant Saccharomyces cerevisiae
其他标题：Conversion of Waste Agriculture Biomass to Bioethanol by Recombinant Saccharomyces cerevisiae
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作者：A. A. Saleh ; S. Hamdan ; N. Annaluru 等
期刊名称：Journal of Scientific Research
印刷版ISSN：2070-0237
电子版ISSN：2070-0245
出版年度：2010
卷号：2
期号：2
页码：351-361
DOI：10.3329/jsr.v2i2.2882
语种：English
出版社：Rajshahi University
摘要：Agricultural waste biomass has already been transferred to bioethanol and used as energy related products, although many issues such as efficiency and productivity still to be overcome. In this study, the protein engineering was applied to generate enzymes with completely reversed coenzyme specificity and developed recombinant yeasts containing those engineered enzymes for construction of an efficient biomass-ethanol conversion system. Recombinant yeasts were constructed with the genes encoding a wild type xylose reductase (XR) and the protein engineered xylitol dehydrogenase (XDH) (with NADP) of Pichia stipitis. These recombinant yeasts were characterized based on the enzyme activity and fermentation ability of xylose to ethanol. The protein engineered enzymes were expressed significantly in Saccharomyces cerevisiae as judged by the enzyme activity in vitro. Ethanol fermentation was measured in batch culture under anaerobic conditions. The significant enhancement was found in Y-ARS strain, in which NADP+-dependent XDH was expressed; 85% decrease of unfavorable xylitol excretion with 26% increased ethanol production, when compared with the reference strain expressing the wild-type XDH. Keywords: Agricultural waste biomass; Protein engineering; Xylitol dehydrogenase; Xylose-fermentation; Eethanol production. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i2.2882 J. Sci. Res. 2 (2), 351-361 (2010)
关键词：Engineering; Biomass;Agricultural waste biomass; Protein engineering; Xylitol dehydrogenase; Xylose-fermentation; Ethanol production.