文章基本信息

标题：Development and Validation of a RP-HPLC Method for the Quantification of Omeprazole in Pharmaceutical Dosage Form
其他标题：Development and Validation of a RP-HPLC Method for the Quantification of Omeprazole in Pharmaceutical Dosage Form
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作者：R. Kayesh ; M. Z. Sultan ; A. Rahman 等
期刊名称：Journal of Scientific Research
印刷版ISSN：2070-0237
电子版ISSN：2070-0245
出版年度：2013
卷号：5
期号：2
页码：335-342
DOI：10.3329/jsr.v5i2.12779
语种：English
出版社：Rajshahi University
摘要：A rapid and highly sensitive reversed phase high performance liquid chromatographic method has been developed for quantitative estimation of omeprazole in pharmaceutical preparations. The method has been validated according to FDA and USP guidelines with respect to accuracy, precision, specificity and linearity. The method was developed by using a gradient condition of mobile phase comprising 90% aqueous acetonitrile to 100% acetonitrile for 10 minutes at a flow rate of 0.7 mL/min over C-18 (ODS, 250 x 4.6 mm) column at ambient temperature. More than 97% recovery demonstrated the accuracy of the protocol. Intra-day and inter-day precision studies of the new method were less than the maximum allowable limit (RSD% £ 2.0 according to FDA). The method showed linear response with correlation coefficient (r2) value of 0.998. Therefore, it was found to be accurate, reproducible, sensitive and less time consuming and can be successfully applied for the assay of omeprazole formulations.Keywords: HPLC; Method development; Gradient condition; Validation; Omeprazole.© 2013 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v5i2.12779 J. Sci. Res. 5 (2), 335-342 (2013)
关键词：Pharmacy;HPLC; Method development; Gradient condition; Validation; Omeprazole.