期刊名称：Revista Internacional de Contaminación Ambiental
印刷版ISSN：0188-4999
出版年度：2010
卷号：4
期号：1
页码：57-63
出版社：Centro de Ciencias de la Atmósfera
摘要：A method for the analysis of sister chromatid exchanges (SCEs) occurring in three subsequent cell divisions in vivo , was developed in murine bone marrow cells. The method was based on the three-way differential staining of sister chromatids. Mice were injected twice i.p. with BrdU adsorbed to activated charcoal; the first dose at 0 h (first cell cycle) was varied between 0.1 and 0.25 mg of BrdU/g of body weight and the second dose at 8 h (second and third cell cycles), was either 1.0, 1.33 or 1.5 mg of BrdU/g of body weight. Animals were injected with colchicine (i.p.) two hours before sacrificing and chromosome prepations were obtained by the usual method. After sister chromatid differentiation by the method of fluorescence plus Giemsa the cells were scored for well and poor differential staining and in some cases for SCE frequency in three subsequent cell divisions. The results indicate that a higher percentage of well-differentiated three way stained cells were obtained with the combination of BrdU doses of 0.2 and 1.5 mg/g of body weight. The very low basal frequency of SCE observed in the first cell cycle (0.31-0.46) probably increases the sensitivity to detected the mutagenic effect by SCE method. This method can be used for both the analysis of in vivo persistence of lesions involved in SCE induction as well as the detection of sinergism between the mutagen action and the BrdU incorporatioa to DNA.
