期刊名称：Revista Internacional de Contaminación Ambiental
印刷版ISSN：0188-4999
出版年度：2010
卷号：3
期号：1
页码：55-61
出版社：Centro de Ciencias de la Atmósfera
摘要：The cytogenetic effects of chromium trioxide (CrO3) in meristematic root tips of Vicia faba were evaluated by the analysis of chromosomal alterations in anaphase cells. The CrO 3 , concentrations of 0.001, 0.01, 0.05, 0.10, 0.50, 1 .O, 1.5, 2.0, 2.5, and 3.0% were applied for 1, 2 and 3 hours without recovery. Longer exposures damaged the tissues. This substance induced chromosomal aberrations and also produced injury in the centromere, leading to chromosomes with centromere inactivated and isochromosomes as well as alterations of the mitotic spindle causing multipolar anaphases and C-metaphases. No relation in concentration-effect was found because the highest frequencies of abnormal anaphases, total aberrations and multipolar anaphases were noted at 0.01% of CrO3, which decreased at higher concentrations. This behavior was possibly due to the fact that the absorbance of high concentrations of chromium by the roots, impregnated its cell walls therefore avoiding the entrance of more chromium. When colchicine was applied simultaneously to the treatment, the higher CrO 3 , concentration the lower number of C-metaphases in relation to anaphases, thus at low CrO 3 , concentrations the colchicine uptake was higher.
