期刊名称:Indian Journal of Innovations and Developments
印刷版ISSN:2277-5382
电子版ISSN:2277-5390
出版年度:2012
卷号:1
期号:9
页码:665-668
出版社:Indian Society for Education and Environment
摘要:Banana plantlets propagated through shoot tip culture were used for in vitro rooting in ½ strength MS medium supplemented with different concentrations (0.2mg/l - 2.0mg/l) of three different auxins either alone or in combinations. ½ strength MS + 1.0 mg/l IBA + 0.5 mg/l IAA induced roots among 90% of the explants after 12 th days of inoculation. The average number of roots in this condition was 6.0. Increase in the concentrations of the above two auxins had no promoting impact; rather the number of roots were reduced. Similarly IBA (1.5mg/l) alone induced rooting but the percentage response was reduced (66%) and time of initiation was increased (18 th days). Lower concentration of IBA (0.5mg/l) + 0.5mg/l IAA had also no promoting impact. About 70% of the explants responded in this after 15th days of incubation. Higher concentration of IBA (1.5mg/l) + 0.5mg/l NAA, revealed reduced rate of response, similarly IBA at its different concentration revealed no better response with NAA at 0.2 or 0.5mg/l.
关键词:Micropropagation, Plantlets, in Vitro, Explants, Auxins, Innoculation