Genetic polymorphic markers such as blood groups are widely used genetic markers in human population genetic studies. This study reports the genetic variation in phenotype and allele frequencies of ABO and Rh (D) blood groups among the Kshatriyas, an endogamous population from Visakhapatnam district, Andhra Pradesh, India. Blood samples of 100 unrelated individuals were screened for ABO and Rh (D) blood groups. The order of occurrence of ABO phenotypes is O>B>A>AB. The corresponding allele frequencies of O, A and B are 0.420, 0.155 and 0.415, respectively. The allele frequency of D (0.990) is more than d (0.010). The present results are compared with the other populations to understand the population variations.