摘要：Introduction. Since human papillomavirus (HPV) is the central casual factor in cervicalcancer, understanding the epidemiology and geographical area distribution of the mostprevalent HPV genotypes constitutes an important step towards development of strategiesof prevention.Aim. The aim of this study was to investigate the prevalence of HPV infection and todetermine HPV types distribution among 822 HPV positive women and some sexualmale partners in Apulia (Italy).Methods. HPV DNA detection and genotyping was performed by nested-PCR for theL1 region and reverse line blot hybridization allowing the specific detection of 24 HPVgenotyping both high risk (HR) and low risk (LR).Results. The most prevalent HPV genotypes were HPV 16 (35%), HPV 31 (16%) HPV6 (9%), HPV 58 and 66 (7%), followed by HPV 33 (6%), HPV 18 and 56 (4%), HPV70 and 45 (3%), HPV 53 and 11 (2%). Currently 1.5% of tested specimens remainedunclassified. Multiple infections with at last two different high- risk HPV genotypes wereobserved in 10% of specimens.Conclusions. This finding adds knowledge to HPV epidemiological investigation, andaddresses further studies aimed to consider public health for identifying groups at riskfor cervical cancer.
关键词：human papillomavirus;(HPV); DNA virus; sexually transmitted;disease; HPV test; Pap smear
