This study explored knowledge, attitudes and practices towards HIV/AIDS infection among 1256 Egyptian industrial and tourism workers aged 16–40 years. Compared with industrial workers, tourism workers had a significantly better perception of the magnitude of the HIV/AIDS problem worldwide as well as in Egypt and of the likelihood of the problem worsening. Knowledge of tourism workers was also significantly better about causative agent of AIDS and methods of transmission. Both groups had negative attitudes towards patients living with HIV/AIDS concerning their right to confidentiality and to work. Both groups had a positive attitude towards behaviour change for protection from HIV/AIDS, principally via avoidance of extramarital sexual relations and adherence to religious beliefs. Use of condoms as a way to avoid HIV/AIDS was reported by only 0.4% of workers.