This study was designed to estimate the burden of urologic diseases in the Islamic Republic of Iran as a practical method for defining public health priorities. Using World Health Organization guidelines for national burden of disease studies, 6 steps were performed selecting diseases to include; dynamic modelling of diseases; gathering data about urologic diseases; computing the local disability weights of urologic diseases; data analysis with DisMod II; and computing the disability-adjusted life years for each disease. Renal calculus, acute cystitis, chronic prostatitis, benign prostate hyperplasia, male infertility and adult polycystic kidney disease comprised the greatest burden.