During the 5-day Eid Al Adha holiday, Aqaba is the site of one of the largest mass gatherings in Jordan. Public health concerns during this holiday included food- and waterborne diseases, drowning, injuries by marine creatures, road traffic crashes, and pressure on emergency departments at hospitals. This cross-sectional study was carried out in Aqaba during the period 16–20 November 2010 and aimed to identify and prioritize the major public health risks and to overview the preparedness plans of the Health Directorate during previous Eid events in Aqaba. All related stakeholders participated in the study. An exploratory visit to Aqaba was made and an introductory workshop was conducted for participants. Relevant data were collected and compared with the figures for the week prior to the event. No food poisoning outbreaks were reported during the event. There was a 23% increase in emergency department attendance, a 33% increase in hospital admissions, and road traffic crashes increased by more than 300%. More males were affected than females.