标题：Effet de prétraitement au froid et au mannitol sur l’androgenèse et la gynogenèse chez des variétés d’orge de printemps (Hordeum vulgare L.) ; Effect of cold and mannitol pretreatment on the androgenesis and gynogenesis in spring barley varieties (Hordeum vulgare L.),
期刊名称：Journal of Materials and Environmental Science
印刷版ISSN：2028-2508
出版年度：2016
卷号：7
期号：7
页码：2583-2594
出版社：University of Mohammed Premier Oujda
摘要：In the context of obtaining haploid plants by in vitro culture of anthers (androgenesis) and unfertilized ovaries (gynogenesis) in barley (Hordeum vulgare) cultivars: Asni and Tamelalt., We studied the effect of cold pretreatment at 4 ° C during 21 days (thermal shock) and 0.7M mannitol for 3 days (osmotic shock). A set of 5221 anthers and 2379 ovaries were cultured on induction medium and regeneration Olsen. The results show the positive effect of cold pretreatment on androgenic induction of calluses and/or embryos and regeneration of plants mostly among the cultivar Tamelalt but with a high proportion of albino plants. In gynogenesis, all plants regenerated from ovaries (without any pretreatment) or pretreated with mannitol were of type chlorophyllous (4 for Tamelalt and 2 of Asni).
关键词：androgenesis; gynogenesis; Hordeum vulgare; in vitro culture; haplodiplo.dization; cold; mannitol
