摘要：Symptoms of organophosphate intoxication are a result of blocking the activity of blood cholinesterase enzyme (ChEA). The ability of liver to do de toxification using pathway of O-Dealchylation on organophosphate and cholinesterase bound and helped out by Glutathione peroxides enzyme (GPX) has important role in returning the level of ChEA to the normal level. GPX is dependent on the presence of selenium. The objective of this study is to obtain more understandable benefit of adding selenium and vitamin C towards the ChEA, GPX and hemoglobin level of farmers worked as pesticide sprayers. This is an experimental study using Pretest-posttest Control Group Design. Ninety nine respondents in Pasuruan village, sub district of Bulu, Temanggung who fulfilled inclusion and exclusion criteria were selected as study samples. Samples were divided into 3 groups of 33 people. First group received only Selenium, second group received Selenium and Vitamin C and third group is a control group (no supplementation given). Blood samples of all study samples were taken to identify the level of ChEA, GPX and hemoglobin before and after experiment Level of ChEA in the first group compared with the third group was significantly different (p=0.05). Similar finding was also found for the level of ChEA in second group compared with third group (p= 0.014). Supplementation of Selenium 200 μg for 7 days among pesticides sprayers farmers increase 1.85% level of ChEA and 2.66% level of hemoglobin. Keywords: Selenium, vit C, level of GPX, ChEA and Hemoglobin. permalink : http://ejournal.undip.ac.id/index.php/mkmi/article/view/2792
