摘要：ABSTRACT This study was aimed to determine the effect of fish oil addition on growth performance and fatty acid composition of eel Anguilla bicolor bicolor . Freswater eel at an initial body weight of 9.90± 0.05 g were maintained in aquarium with volume of 120 L at stocking density of 1 g/L for 40 days. This research applied complete randomized design with four treatments addition of fish oil, i.e. 0%, 5%, 10%, and 15%. All treatments were carried out in triplicate. Feed given as much as 3% of the fish biomass a day for four times at 06:00, 11:00, 16:00, and 21:00. The results showed that the addition of fish oil in the diet have different effects (P<0.05) on specific growth (0.88–1.36%), feed efficiency (30.18–48.53%), protein retention (14.57–20.24%), fat retention (16.77–52.49%), energy retention (12.38–20.20%), and hepatosomatic index (1.72–2.72%) whereas the survival showed no difference (P>0.05) at 100%. In the fatty acid composition total of unsaturated fatty acid composition was 30.91–40.95%, n-3 fatty acids was 6.10–8.19%, and n-6 fatty acids were 6.18–8.19%. In conclusion, the addition of fish oil in the diet of freshwater eel Anguilla bicolor bicolor can be done up to 5% (13% fat content of diet). Keywords: Anguilla bicolor bicolor , fish oil, growth performance, fatty acid composition ABSTRAK Penelitian ini bertujuan untuk menguji pengaruh penambahan minyak ikan terhadap kinerja pertumbuhan dan komposisi asam lemak ikan sidat Anguilla bicolor bicolor . Ikan sidat dengan bobot 9,90±0,05 g dipelihara dalam akuarium dengan volume 120 L pada padat tebar 1 g/L selama 40 hari. Penelitian ini menggunakan rancangan acak lengkap terdiri atas empat perlakuan penambahan minyak ikan pada pakan sebesar 0%, 5%, 15%, dan 15%. Semua perlakuan terdiri atas tiga ulangan. Pakan diberikan sebanyak 3% dari biomassa ikan dan diberikan sebanyak empat kali sehari yaitu pukul 06.00, 11.00, 16.00 dan 21.00. Hasil penelitian menunjukkan bahwa penambahan minyak ikan dalam pakan memberikan pengaruh yang berbeda (P<0,05) terhadap spesific growth rate (0,88– 1,36%), efisiensi pakan (30,18–48,53%), retensi protein (14,57–20,24%), retensi lemak (16,77–52,49%), retensi energi (12,38–20,10%), dan indeks hepatosomatik (1,72–2,72%). Sintasan tidak menunjukkan adanya perbedaan (P>0,05) yaitu 100%. Pada komposisi asam lemak dihasilkan total komposisi asam lemak tidak jenuh 30,91– 40,95%, asam lemak n-3 6,10–8,19%, dan asam lemak n-6 6,18–8,19%. Dengan demikian, dapat disimpulkan bahwa penambahan minyak ikan dalam pakan ikan sidat Anguilla bicolor bicolor dapat dilakukan sampai dengan 5% (kadar lemak pakan 13%). Kata kunci: Anguilla bicolor bicolor , minyak ikan, kinerja pertumbuhan, komposisi asam lemak
