期刊名称：Walailak Journal of Science and Technology (WJST)
印刷版ISSN：2228-835X
出版年度：2011
卷号：4
期号：1
页码：53-66
语种：English
出版社：Institute of Research and Development, Walailak University.
摘要：Translationally controlled tumour proteins (TCTP) cDNA were cloned from the haemolymph of Scylla paramamosain by reverse transcription PCR using primers derived from Penaeus monodon . Nucleotide sequence analysis revealed 507 bp open reading frame (ORF) encoding 168 amino acid residues with a predicted molecular mass and pI of approximately 19.2 kDa and 4.5, respectively. Interestingly, mud crab-TCTP shows 99 % homology with Penaeus monodon translationally controlled tumour proteins (Pm-TCTP) and Fenneropenaeus merguiensis TCTP. This gene was also expressed in pQE40 vector as histidine tagged fusion proteins. The recombinant protein has a molecular mass of approximately 25 kDa. From the biological function study of Pm-TCTP, It is proposed that mud crab-TCTP might function as anti-apoptosis like Pm-TCTP does.
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