期刊名称：Walailak Journal of Science and Technology (WJST)
印刷版ISSN：2228-835X
出版年度：2016
卷号：13
期号：10
页码：827-835
DOI：10.14456/vol13iss7pp%p
语种：English
出版社：Institute of Research and Development, Walailak University.
摘要：For the purpose of discovering anti-HIV-1 agents from Thai plant preparations with a history of use in Thai traditional medicine as agents assisting health longevity, aqueous and ethanol extracts from 24 Thai plants were screened for their inhibitory activities against HIV-1 protease (PR) using an anti-HIV-1 PR assay. Five extracts (10 %), 3 ethanol and 2 water, from only 4 plants, Cassia garrettiana, Dioscorea bulbifera, Albizia procera , and Areca catechu , produced extracts with IC 50 values of < 45 µg/ml. Thirty five extracts (90 %) had no detectable activity, with IC 50 values >100 µg/ml. The ethanol extracts of Cassia garrettiana (heartwood), Areca catechu , and Dioscorea bulbifera were the most potent, with IC 50 values of 15.6, 24.9, and 44.4 µg/ml, respectively. For the water extracts, the most potent activity was found in Albizia procera (bark) and Cassia garrettiana (heartwood), with IC 50 values of 22.6 and 26.8 µg/ml, respectively. Pure compounds from ethanol extracts of Cassia garrettiana (heartwood) were separated to give 5 compounds, chrysophanol ( 1 ), piceatannol ( 2 ), aloe-emodin ( 3 ), emodin ( 4 ), and cassigarol E ( 5 ). Of the tested samples, piceatannol ( 2 ) showed the highest anti-HIV-1 PR activity (IC 50 = 48.29 µM), whereas the % inhibition (mean±S.E.M., n=3) produced by 100 µM of cassigarol E ( 5 ), aloe-emodin ( 3 ), chrysophanol ( 1 ), and emodin ( 4 ) were only 27.64±3.8, 27.20±0.7, 26.66±0.6, and 4.52±1.9, respectively.
其他摘要：For the purpose of discovering anti-HIV-1 agents from Thai plant preparations with a history of use in Thai traditional medicine as agents assisting health longevity, aqueous and ethanol extracts from 24 Thai plants were screened for their inhibitory activities against HIV-1 protease (PR) using an anti-HIV-1 PR assay. Five extracts (10 %), 3 ethanol and 2 water, from only 4 plants, Cassia garrettiana, Dioscorea bulbifera, Albizia procera , and Areca catechu , produced extracts with IC 50 values of < 45 µg/ml. Thirty five extracts (90 %) had no detectable activity, with IC 50 values >100 µg/ml. The ethanol extracts of Cassia garrettiana (heartwood), Areca catechu , and Dioscorea bulbifera were the most potent, with IC 50 values of 15.6, 24.9, and 44.4 µg/ml, respectively. For the water extracts, the most potent activity was found in Albizia procera (bark) and Cassia garrettiana (heartwood), with IC 50 values of 22.6 and 26.8 µg/ml, respectively. Pure compounds from ethanol extracts of Cassia garrettiana (heartwood) were separated to give 5 compounds, chrysophanol ( 1 ), piceatannol ( 2 ), aloe-emodin ( 3 ), emodin ( 4 ), and cassigarol E ( 5 ). Of the tested samples, piceatannol ( 2 ) showed the highest anti-HIV-1 PR activity (IC 50 = 48.29 µM), whereas the % inhibition (mean±S.E.M., n=3) produced by 100 µM of cassigarol E ( 5 ), aloe-emodin ( 3 ), chrysophanol ( 1 ), and emodin ( 4 ) were only 27.64±3.8, 27.20±0.7, 26.66±0.6, and 4.52±1.9, respectively.
