期刊名称：Walailak Journal of Science and Technology (WJST)
印刷版ISSN：2228-835X
出版年度：2015
卷号：13
期号：2
页码：133-140
语种：English
出版社：Institute of Research and Development, Walailak University.
摘要：Andrographis paniculata leaf aqueous extract over 0.5, 1, 3, 5, and 24 h were determined to contain the highest amount of total phenolic compound and was used to evaluate mosquito larvicidal properties against Culex quinquefasciatus at varies concentrations (3.125, 6.25, 12.5, and 25 ppm) by determining the median and 90 % lethal concentration, LC 50 and LC 90 , respectively, within 24 h and by histological analysis. The results revealed that the total phenolic compound measurement in each time extraction were 7.97, 8.68, 7.16, 6.70, and 5.78 mg/g GAE, respectively. The one hour aqueous extract of A. paniculata leaf expressed the 24-h LC 50 and LC 90 values in C. quinquefasciatus which were 15.93 and 28.23 ppm, respectively. Dose dependent lesions were observed. Under histological analysis, midgut lesions, i.e., separation of the epithelial cells from the basement membrane, elongation protruding into the lumen, disruption of the brush border, and the appearance of several vesicles and cytoplasm masses were observed in this study. The present study reveals that the aqueous extract of this leaf has a suitable property for a larvicidal natural product, and may replace harmful chemical pesticides. doi: 10.14456/WJST.2016.14
