An increasing growth in the number of e-learning sites at universities and other educational institutions led to necessary of develop and adopt a standards element to assess these sites to ensure efficiency, rival, and educational quality. Therefore, this study proposed an assessment criterion to evaluate the e-learning sites as a guide for decision-makers in order to purchase and development e-learning sites in which these assessment criterions are commensurate with the learning process. An experts’ opinion from universities professors who specialize in the field of teaching in different Jordan universities have been considered in order to develop a proposed assessment criterion, the result shows that the assessment criterion to evaluate the e-learning sites has twenty six criterion under five main categorizes namely: website design, and scientific knowledge content, technical elements, operational elements and finally with credibility of information sites. Given that the proposed assessment criterion to evaluate e-learning sites is guide for students, teachers, owners and developers about the benefits of e-learning sites.