Quality of instructional performance is the key skill needed for every teaching faculty for achieving the level of pedigree in the present educational scenario. However, the truth is that there are minimal studies to analyze the competency linking emotional intelligence to quality of instructional performance. Therefore the present attempt is to study the role of E.I. among University Instructors and analyze their quality of instructional performance. A sample of 110 Professors participated in the survey from different Universities in the Emirates of Abu Dhabi and Al-Ain. Emotional Quotient Inventory scale was developed to measure the EQ of Instructors. Statistical technique like ‘t’ test and ANOVA was used to find the test mean difference between two groups and more than two groups. The result revealed that emotional intelligence and self-efficacy had very significant relationship towards their work attitude followed by their performance.