The research investigated into the operations and activities of the sub-district structures of local government in Ghana. Three districts in the Asante Region were studied using a cross-sectional study design. Data were collected from both primary and secondary sources. In addition to literature review, a sample size of 79 was used and responses from mainly the Chairmen of the sub-district structures represented the primary data. Data were analysed using both quantitative and qualitative techniques. Results from the analysis indicated that sub-district structures are confronted with a number of constraints that militate against the realization of their potential for inducing grassroots development. The constraints include: poor or no office accommodation, lack of commitment from district assemblies and sub-metropolitan units to provide the needed assistance to the sub-district structures. However, the Unit Committees representing the last tier of the local government structure are more effective and efficient in keeping touch with the grassroots than the Town, Area, Zonal and Urban councils. Recommendations are made to the Local Government Ministry but worthy of note among the recommendations are the urgent need to officially inaugurate all sub-district structures that have not been inaugurated and initiate a process to review the Legislative Instrument establishing Sub-district structures in Ghana.