This study was carried out to solve the problem of the ice-ice disease faced by seaweed farmers. The purpose of this research was to describe the effect of ice-ice disease knowledge and seaweed farming knowledge to cutivation behavior; the effect of ice-ice disease knowledge, and the seaweed cultivation knowledge to the cultivation motivation; and the effect of cultivating motivation to the cutivation behavior of the seaweed farmers. This research is a correlational study to look at the relationships between exogenous with endogenous variables. The study involved 60 seaweed farmers in Tablolong village. The data of this study consists of cultivation behavior and motivation collected by questionnaire, the data about knowledge of ice-ice disease, and seaweed cultivation gathered through the knowledge test sheet. Data were analyzed by path analysis through SPSS for Window.The results showed that there is a direct effect of the ice-ice disease knowledge toward the cultivation motivation but not for cultivation knowledge; and there is a direct effect of the cultivation motivation to the cultivation behavior. It can be concluded that the ice-ice knowledge directly effect toward cultivation motivation and the cultivation motivation effect toward behavior of the seaweed farmers in Kupang district.