期刊名称：Journal of Environmental Science and Technology
印刷版ISSN：1994-7887
电子版ISSN：2077-2181
出版年度：2017
卷号：10
期号：1
页码：13-24
DOI：10.3923/jest.2017.13.24
出版社：Asian Network for Scientific Information
摘要：Background and Objective: The soil samples were collected from the pesticides contaminated sites of different agricultural farms in Sivagangai district, Tamilnadu, India. The present study has been worked microbial and enzymatic for the degradation of monocrotophos (MCP) in soil of Agricultural farms. Bacterial transformations have been the main focus of our study on monocrotophos pesticide degradation. Methodology: Physico- chemical properties of monocrotophos (MCP) contaminated soil samples were analyzed. Enrichment techniques were used to isolate the monocrotophos degrading microbes. Preliminary screenings of monocrotophos degrading bacterial isolates were analyzed, such as screening for phosphatase activity, detection of ammonia, enzyme esterase detection. Effects of different concentrations of MCP in degradation process were measured. The degradation experiment was carried out by inoculating the MSM medium with different concentration of MCP. The MCP degradation by these isolates and their equimolar mixture were confirmed by using analytical tools, fourier transform infrared spectroscopy (FTIR) for 15th, 30th and 45th day. Results: Site 1, 4 and 8 have the higher amount of macro and micro nutrient levels. Totally 20 different bacterial strains were isolated using mineral salt medium containing MCP as a carbon and phosphorus source by enrichment method. About 25% of isolates have the ability to produce phosphatase activity and 35% of Isolates have both ammonia and esterase enzyme production. Based on the MCP tolerance, exhibited in gradient plate assay the isolates BAGN005, BKGN007 and BVGN010 were identified as Bacillus subtilis , Bacillus licheniforms and Pseudomonas stutzeri , respectively. The results of this study demonstrated that BAGN005, BKGN007 and BVGN010 and mixed culture were hydrolase the peaks at 2649.19 and 2115.72 cm1 which indicates MCP degradation and selected for further study as efficient candidates for bioaugmentation of MCP contaminated soil environments. Conclusion: In this study, it reports on monocrotophos degrading bacterial Isolates that have been proven as the promising agent for bioremediation of pesticide polluted environments. The indigenous strains Bacillus subtilis (BAGN005), Bacillus licheniforms (BKGN007) and Pseudomonas stutzeri (BVGN010) were quite efficient in degrading the MCP. It is the first study on MCP degrading capabilities of bacterial species Bacillus tequilensis , as for as our knowledge goes.
