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  • 标题:Identification of Nematode Trapping Fungus Monacrosporium eudermatum Based on Genetic Diversity Using RAPD Technique
  • 本地全文:下载
  • 作者:Ali A. Kasim ; Maitham Dragh
  • 期刊名称:American Journal of Microbiological Research
  • 印刷版ISSN:2328-4129
  • 电子版ISSN:2328-4137
  • 出版年度:2016
  • 卷号:4
  • 期号:6
  • 页码:178-180
  • DOI:10.12691/ajmr-4-6-4
  • 出版社:Science and Education Publishing
  • 摘要:Nematode-trapping fungus Monacrosporium eudermatum a predacious fungus of nematodes, has been very useful in understanding the most relationship between nematophagous fungi and their nematode hosts. M.eudermatum is by far the common nematode-trapping fungus with the characteristic ability of forming adhesive trapping nets once in contact with nematodes. Total DNA was extracted from M.eudermatum sera and amplified by RAPD-PCR using three random primers (OPA-16, OPB-08, OPF-05). The results show amplification of all markers (OPF-05, OPB-08, OPA-16) with the genomic DNA studied. As evidenced by the results, all the markers covered in the study showed high polymorphism (up to 60%) with the exception of the primer (OPB-08) which showed no polymorphism.
  • 关键词:nematophagous; Monacrosporium eudermatum; RAPD technique; PCR
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