期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2016
卷号：113
期号：46
页码：13069-13074
DOI：10.1073/pnas.1602393113
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：SignificancePrimary cilia are immotile organelles composed of specialized structural and signaling proteins that are essential for Hedgehog (HH) signaling. cAMP-dependent protein kinase (PKA) critically regulates HH signaling, maintaining transcriptional repression. Ciliary PKA activity is critical for HH regulation; however, the signaling steps regulating PKA activity within cilia remain unclear/unexplored. Targeted sensors were used to measure signaling molecules specifically within cilia. We found that cilia have basally high cAMP and PKA activity, controlled by phosphatidylinositol (3,4,5)-trisphosphate, not G proteins. Sonic Hedgehog stimulation reduces ciliary cAMP and PKA activity, which is dependent on Ca2+ dynamics. Protein kinase A (PKA) phosphorylates Gli proteins, acting as a negative regulator of the Hedgehog pathway. PKA was recently detected within the cilium, and PKA activity specifically in cilia regulates Gli processing. Using a cilia-targeted genetically encoded sensor, we found significant basal PKA activity. Using another targeted sensor, we measured basal ciliary cAMP that is fivefold higher than whole-cell cAMP. The elevated basal ciliary cAMP level is a result of adenylyl cyclase 5 and 6 activity that depends on ciliary phosphatidylinositol (3,4,5)-trisphosphate (PIP3), not stimulatory G protein (Gs), signaling. Sonic Hedgehog (SHH) reduces ciliary cAMP levels, inhibits ciliary PKA activity, and increases Gli1. Remarkably, SHH regulation of ciliary cAMP and downstream signals is not dependent on inhibitory G protein (Gi/o) signaling but rather Ca2+ entry through a Gd3+-sensitive channel. Therefore, PIP3 sustains high basal cAMP that maintains PKA activity in cilia and Gli repression. SHH activates Gli by inhibiting cAMP through a G protein-independent mechanism that requires extracellular Ca2+ entry.
