首页    期刊浏览 2024年11月25日 星期一
登录注册

文章基本信息

  • 标题:Gram-positive bacteria are held at a distance in the colon mucus by the lectin-like protein ZG16
  • 本地全文:下载
  • 作者:Joakim H. Bergström ; George M. H. Birchenough ; Gergely Katona
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:2016
  • 卷号:113
  • 期号:48
  • 页码:13833-13838
  • DOI:10.1073/pnas.1611400113
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:SignificanceThe small lectin-like protein ZG16 (zymogen granulae protein 16) aggregates bacteria and by that works together with the inner colon mucus layer to maintain bacteria at a safe distance from the epithelial cell surface. In the absence of Zg16, more bacteria penetrate the epithelium, enter the regional lymph nodes and spleen, trigger the immune system, and cause abdominal fat pad mass increase. ZG16 is important for a safe normal host-bacteria symbiosis as it does not kill commensal bacteria, but limit bacterial translocation into the host. The distal colon functions as a bioreactor and harbors an enormous amount of bacteria in a mutualistic relationship with the host. The microbiota have to be kept at a safe distance to prevent inflammation, something that is achieved by a dense inner mucus layer that lines the epithelial cells. The large polymeric nets made up by the heavily O-glycosylated MUC2 mucin forms this physical barrier. Proteomic analyses of mucus have identified the lectin-like protein ZG16 (zymogen granulae protein 16) as an abundant mucus component. To elucidate the function of ZG16, we generated recombinant ZG16 and studied Zg16-/- mice. ZG16 bound to and aggregated Gram-positive bacteria via binding to the bacterial cell wall peptidoglycan. Zg16-/- mice have a distal colon mucus layer with normal thickness, but with bacteria closer to the epithelium. Using distal colon explants mounted in a horizontal perfusion chamber we demonstrated that treatment of bacteria with recombinant ZG16 hindered bacterial penetration into the mucus. The inner colon mucus of Zg16-/- animals had a higher load of Gram-positive bacteria and showed bacteria with higher motility in the mucus close to the host epithelium compared with cohoused littermate Zg16+/+. The more penetrable Zg16-/- mucus allowed Gram-positive bacteria to translocate to systemic tissues. Viable bacteria were found in spleen and were associated with increased abdominal fat pad mass in Zg16-/- animals. The function of ZG16 reveals a mechanism for keeping bacteria further away from the host colon epithelium.
  • 关键词:mucin ; colon ; inflammation ; obesity ; peptidoglycan
国家哲学社会科学文献中心版权所有