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  • 标题:Differential control of retrovirus silencing in embryonic cells by proteasomal regulation of the ZFP809 retroviral repressor
  • 本地全文:下载
  • 作者:Cheng Wang ; Stephen P. Goff
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:2017
  • 卷号:114
  • 期号:6
  • 页码:E922-E930
  • DOI:10.1073/pnas.1620879114
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Replication of the murine leukemia viruses is strongly suppressed in mouse embryonic stem (ES) cells. Proviral DNAs are formed normally but are then silenced by a large complex bound to DNA by the ES cell-specific zinc-finger protein ZFP809. We show here that ZFP809 expression is not regulated by transcription but rather by protein turnover: ZFP809 protein is stable in embryonic cells but highly unstable in differentiated cells. The protein is heavily modified by the accumulation of polyubiquitin chains in differentiated cells and stabilized by the proteasome inhibitor MG132. A short sequence of amino acids at the C terminus of ZFP809, including a single lysine residue (K391), is required for the rapid turnover of the protein. The silencing cofactor TRIM28 was found to promote the degradation of ZFP809 in differentiated cells. These findings suggest that the stem cell state is established not only by an unusual transcriptional profile but also by unusual regulation of protein levels through the proteasomal degradation pathway.
  • 关键词:embryonic stem cells ; retrovirus ; transcriptional repressor ; proteasome
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