期刊名称：Advance Journal of Food Science and Technology
印刷版ISSN：2042-4868
电子版ISSN：2042-4876
出版年度：2016
卷号：11
期号：12
页码：765-769
DOI：10.19026/ajfst.11.2789
出版社：MAXWELL Science Publication
摘要：To develop an immunemagnetic-bead-based enzyme-linked immunesorbent assay (IMB-ELISA), antisera were prepared12 from New Zealand white rabbits immunized with inactivated Pseudomonas fluorescens ( P. fluorescens ) and conjugated with magnetic-bead to get immunomagnetic-beads. IMB-ELISA would be developed through IMB collecting P. fluorescens . The results showed that the limit of detection ELISA and IMB-ELISA are, respectively 101.1 cfu/mL and 101.0 cfu/mL. The IMB-ELISA is more sensitive and higher efficient than IC-ELISA by calculating the recovery rate and the coefficient of variation, because of IMB quickly enriching the target bacteria. The IMB-ELISA is a quick and accurate method for the detection of P. fluorescens .
