期刊名称：Advance Journal of Food Science and Technology
印刷版ISSN：2042-4868
电子版ISSN：2042-4876
出版年度：2016
卷号：11
期号：12
页码：800-804
DOI：10.19026/ajfst.11.2795
出版社：MAXWELL Science Publication
摘要：Rhodobacter sphaeroides is abundant in natural carotenoids. However, fermentation under light growth conditions for carotenoids biosynthesis is time consuming. In the present study, fermentation for 36 h under micro-aerobic growth conditions was employed to rapidly produce carotenoids from Rb. Sphaeroides . Orthogonal experiments suggested that the optimal fermentation process was: temperature 33°C, inoculation amount 8%, fermentation duration 36 h. The yield of total carotenoids was 11.542 mg/L. Real-time PCR indicated that expression levels for crtA , crtD , crtE and crtI at the time fermentation for 36 h were higher than that of fermentation for 48 h. TLC analysis showed that at least 6 well separated bands were observed. Temperature, pH and light tolerance tests indicated that the total carotenoids were stable. 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) assay suggested that the total carotenoids exhibited high anti-oxidant activity and the IC50 value of DPPH radical scavenging test was about 8.175 μg/mL. The present study will promote to produce natural carotenoids from microorganism in large-scale.
