期刊名称：Advance Journal of Food Science and Technology
印刷版ISSN：2042-4868
电子版ISSN：2042-4876
出版年度：2016
卷号：12
期号：8
页码：451-456
DOI：10.19026/ajfst.12.2999
出版社：MAXWELL Science Publication
摘要：In order to understand the hypolipidemic mechanism of steviosides from Stevia rebaudiana leaves, we optimised ratio of solid to liquid, extraction temperatures, extraction times, time to obtain optimal extraction conditions. Under the optimal condition (solid to liquid 1:20, extraction temperature 80C, and extraction times 4h, time 4), the experimental yield of steviosides was gave the best results (8.637±0.27%). The SVS-2 were obtained by a DEAE-52 cellulose column and Sephadex G-15 column chromatography. The SVS-2 was characterised through FT-IR spectroscopy, NMR spectroscopy, HPLC-PAD-MS analysis by comparison with a stevioside standard. In vitro test, the steviosides can decrease liver TC, TG and serum TG, TC level and increased swimming time of rats fed with high-fat diets. Therefore, the results suggest that steviosides had a high hypolipidemic activity and could be used as a potential therapeutic agent for hyperlipidemia.
