摘要：Hepatitis B infection affects individuals worldwide, especially in Latin America. Serological assay for HBV antibodies and antigen detection, are critical for HBV diagnostic and treatment. Available commercial ELISA kits are expensive and in our country not always are readily available. The aim of this study was develop a homemade ELISA kit for serological detection IgG anti-HBV core (IgG anti-HBc) accessible for our patients. 114 samples were analyzed: 17 from seronegatives individuals, 48 from seropositives patients and 49 belonging to indigenous population from Mérida State. Exposure to Hepatitis B virus was determined using the Murex anti-HBc test (DiaSorin, UK). Homemade ELISA shows 100% specificity and del 100 % sensitivity, and was in very good agreement with serological status, Kappa= 1 (CI 95%: 0,767-1), X2 (p<0,001). In indigenous population (Wayuu and mestizos), overall IgG anti-HBc prevalence was 22%. Taking into account these results, homemade IgG anti-HBc ELISA is efficacious and low cost usefully, for HBV diagnosis.
关键词：Hepatitis B core;homemade assay;Wayuu;mestizos;Hepatitis B virus;Hepatitis B core;prueba casera;Wayuu;mestizos;virus de la Hepatitis B
