期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1986
卷号：1
期号：1
页码：5-10
DOI：10.3164/jcbn.1.5
出版社：The Society for Free Radical Research Japan
摘要：As a chemiluminescence probe, 2-methyl-6-phenyl-3, 7dihy-droimidazo[1, 2-a]pyrazin-3-one was used to detect superoxide anion generation in granulocytes during phagocytosis. The reaction of this probe is highly specific for and sensitive to superoxide anions and can be adopted to determine them in terms of xanthine oxidase units. Generation of superoxide anions in response to opsonized zymosan was checked for granulocytes of peripheral blood samples from 9 adults and those of cord blood samples from 11 neonates. The ability to generate superoxide anions during phagocytosis was found to be greater in granulocytes of cord blood from neonates than in those of peripheral blood from adults.
