首页    期刊浏览 2024年12月01日 星期日
登录注册

文章基本信息

  • 标题:Use of Phospholipase D and Choline Oxidase for the Enzymatic Determination of Calcium Ion in Serum
  • 本地全文:下载
  • 作者:Masayoshi TABATA ; Takahiro KIDO ; Masaki IKEMOTO
  • 期刊名称:Journal of Clinical Biochemistry and Nutrition
  • 印刷版ISSN:0912-0009
  • 电子版ISSN:1880-5086
  • 出版年度:1986
  • 卷号:1
  • 期号:1
  • 页码:11-19
  • DOI:10.3164/jcbn.1.11
  • 出版社:The Society for Free Radical Research Japan
  • 摘要:A novel enzymatic method for the determination of calcium ion (Ca2+) is described. The principle of the assay is to determine the degree of activation by Ca2+ present in a given solution of a Ca2+-dependent enzyme, phospholipase D, which is subsequently assessed in terms of the amount of choline produced. For the latter determination, choline oxidase and peroxidase are used in combination, leading to the color production, which can be simply quantified by measuring the increase in absorbance at 500nm with time. The present method is highly Ca2+-specific, accurate, and reproducible, giving results in reasonably good agreement with those obtained by the Ca2+-selective electrode method. The results of the enzymatic method are not influenced by any change in pH of the serum samples that may occur during the storage of these samples.
  • 关键词:calcium ion;phospholipase D;choline oxidase;Ca2+ in serum;enzymatic assay
国家哲学社会科学文献中心版权所有