期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1991
卷号：10
期号：1
页码：21-25
DOI：10.3164/jcbn.10.21
出版社：The Society for Free Radical Research Japan
摘要：We previously constructed novel liposomes for efficient transfection of an entrapped gene into mammalian cells [Koshizaka, T., Hayashi, Y., and Yagi, K. (1990) J. Clin. Biochem. Nutr ., 7, 185-192]. By encapsulation of histone, a nuclear protein, into the above liposomes at a histone/DNA ratio (w/w) of 0.2, the expression in COS-1 cells of β-galactosidase activity by the plasmid pCH110, which contains the lacZ gene under the control of the SV40 early promoter, was found to be increased by -50%. The increased expression efficiency seems to be due to the efficient transfer of the plasmid to the nucleus after its incorporation into the cells, probably through the protection of the DNA by its binding with histone and/or accelerated transfer of the DNA to the nucleus by this protien.
