期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1991
卷号：10
期号：2
页码：119-126
DOI：10.3164/jcbn.10.119
出版社：The Society for Free Radical Research Japan
摘要：Feeding of ellagic acid, one of the naturally occurring plant phenols, at varying dose levels through drinking water for eight weeks resulted in a dose-dependent significant increase in the hepatic glutathione-S-transferase activity as well as in the hepatic and pulmonary reduced glutathione levels. However, the pulmonary glutathione-S-transferase activity was not affected. Glutathione-S-transferase inhibition curve, obtained at different concentrations of ellagic acid in the 10, 000× g hepatic supernatant using 1-chloro-2, 4-dinitrobenzene as substrate, suggests two types of isoenzymes of glutathione-S-transferase, ellagic acid-binding (isoenzymes to which ellagic acid is prone to bind) and ellagic acid-non binding (isoenzymes to which ellagic acid does not bind). I50 for ellagic acid-binding isoenzymes was found to be 35μM. Kinetic studies revealed that ellagic acid inhibited glutathione-S-transferase activity non-competitively with respect to 1-chloro-2, 4-dinitro-benzene as a substrate, with a K i of 450μM and K s of 322μM.
