期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1992
卷号：12
期号：3
页码：153-160
DOI：10.3164/jcbn.12.153
出版社：The Society for Free Radical Research Japan
摘要：We developed a new approach to the treatment of malignant glioma by intracellular production of human β-interferon (HuIFN-β). In our previous study [Mizuno, M., et al. (1990): Cancer Res., 50, 7826-7829], we could inhibit the growth of cultured glioma cells by transfecting them with the HuIFN-β gene by means of novel liposomes bearing a monoclonal antibody against a glioma-associated surface antigen. To kill the cells, however, some further device was needed. In the present study, we found by in vitro experimentation that production of HuIFN-β in the cells transfected with the gene could be increased by treatment of the cells with tumor necrosis factor-α (TNF-α) prior to the transfection and that the cells completely disappeared by 9 days after the transfection. Human glioma subcutaneously transplanted into nude mice could be killed completely if the mice were given TNF-α prior to the gene transfection. Thus the gate to gene therapy for brain tumors has been opened.
